STUDIES OF NATIVE GLYCOGEN ISOLATED FROM SYNCHRONIZED TETRAHYMENA PYRIFORMIS (HSM)
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چکیده
منابع مشابه
Studies of Native Glycogen Isolated from Synchronized Tetrahymena Pyriformis (hsm)
Native glycogen was isolated from Tetrahymena pyriformis (HSM) by isopycnic centrifugation in cesium chloride density gradients. A density of 1.62 to 1.65 was isopycnic for glycogen. Most of the banded glycogen existed as 35 to 40 mmicro particles which had a sedimentation coefficient of 214. These particles were composed of aggregates of 2 to 3 mmicro spherical particles. Extraction of glycoge...
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Cilia isolated from Tetrahymena after ciliary shedding is induced by the addition of CaC1 2 or other salts to cells suspended in an ethanol solution (4, 6, 11) have been studied chemically and enzymatically (4, 6, 1i, 12, 13), but until now have never been shown to be capable of motility in the presence of ATP. In contrast to this, Gibbons (7) has demonstrated the reactivation of glycerolisolat...
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Pellicular fragments were isolated from ethanol-fixed cells of the holotrichous ciliate Tetrahymena pyriformis by the action of digitonin. The isolated pellicles were further fragmented and the basal bodies of the cilia isolated from them by three methods. The preparations, examined in the electron microscope as embedded sections or negatively stained samples, consisted mainly of somewhat defor...
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The ability of Tetrahymena pyriformis to undergo synchronous division following release of inhibition with vinblastine was examined. The degree of synchrony was shown to be correlated with the period of time spent under the influence of vinblastine. Cells were inhibited for different periods of time with vinblastine and then washed free of the inhibitor with fresh medium. The increase in cell n...
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A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.27.2.281